梁 友¹，倪 琦²，王印庚¹，刘志伟^1,3，陈 君^1,4，庞尊方⁵，孙芳芳⁵，马文辉⁵

(1 中国水产科学研究院黄海水产研究所，农业部海洋渔业资源可持续利用重点开发实验室，山东 青岛266071；
2 中国水产科学研究院渔业机械仪器研究所，上海200092；3 博兴县水产局，山东 博兴 256500；
4 上海海洋大学，上海201306；5 莱州明波水产有限公司，山东 莱州261418)

摘 要：研究使用外源激素诱导法所得的云纹石斑雄鱼和雌鱼作为试验用鱼。亲鱼经营养强化后，进行人工催产和采卵授精，受精率达68.5%~83.2 %。研究结果表明该鱼受精卵在水温20~22℃、盐度28~31‰、pH 7.9~8.0的外界水环境下, 44 h后孵化出仔鱼，孵化率为76%~91%；初孵仔鱼经56 d培育，完全变态成为幼鱼。在试验中收集到人工授精上浮好卵（受精24 h后）9.6kg，共获>3 cm的云纹石斑鱼苗23万尾，成活率为1.5%。本研究结果将为云纹石斑鱼在我国大规模人工繁育提供数据和奠定基础。
关键词：云纹石斑；人工繁殖 ；苗种培育

Research on scale manual reproduction techniques of Epinephelus moara
LIANG You ¹, NI QI ², WANG Yin-geng ¹, LIU Zhi-wei ³, CHEN Jun ^1,4, PANG Zun-fang ⁵, SUN Fang-fang ⁵,MA Wen-hui ⁵
(1 Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071，China;
2 Fishery Machinery and Instrument Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200092, China;
3 Bo Xing Aquatic Products Bureau, Boxing 256500，China;
4 Shanghai Ocean University, Shanghai 201306, China ;
5 Laizhou Mingbo Fisheries Company LTD., YanTai 265600, China )

Abstract: The paper illustrates the studies on manual reproduction techniques of Epinephelus moara. Parent fish through enriching nutriments, artificially induced spawning and fertilizing, the fertilization was 68.5%~83.2%. After 44h, the fertilized eggs hatched out under conditions of water temperature 20~22℃, salinity28~31, pH7.9~8.0.And the hatching rate was 76%~91%. After 56 days, the larvae metamorphosed into juvenile. After 24h fertilization, 9.6kg floating fertilized eggs were collected and 230 thousand fries of Epinephelus moara over 3cm of total length were produced. The survival rate was 1.5%.
Key words: Epinephelus moara ; Artificial propagation; Larval rearing

基金名称：国家鲆鲽类产业技术体系（nycytx-50）
作者简介：梁友（1964—），男，副研究员，主要从事海水养殖及设施渔业专业，E-mail：liangyou@ysfri.ac.cn
 

（《渔业现代化》2011.38（5）：31-34）